Kit Contents

Anti-Bovine IgG coated strip plate   (8 wells x 12 strips) 80112. Bovine IgG Std. A (0 ng/ml), 0.25 ml 80123.   Bovine IgG Std. B (25 ng/ml), 0.25 ml 80134. Bovine IgG Std. C (50 ng/ml), 0.   25 ml 80145. Bovine IgG Std. D (100 ng/ml), 0. 25 ml 80156. Bovine IgG Std. E   (250 ng/ml), 0. 25 ml 80167. Bovine IgG Std. F (500 ng/ml), 0. 25 ml 80178.   Bovine IgG Std. G (1000 ng/ml), 0. 25 ml 80189. Sample Diluent (20x), 10ml   SD-20B10. Wash Buffer (100X), 10 ml WB-10011. Anti-Bovine IgG-HRP Conjugate   (100x), 0.12 ml 801912. TMB Substrate, 12 ml 8009113. Stop solution, 12 ml   8010114. Instruction Manual M-8010

TMB (substrate), H2SO4 (1% in a   buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample   diluent and HRP-conjugates

Samples

Serum. Plasma, Culture medium and   other fluids may be adapted

Crossreactivity

Bovine IgG ELISA kit cross   reactivity was tested with the following animal sera (IgG) at dilutions of   1:10k: Significant crossreactivity was observed with Rabbit (2.2%) serum IgG.   Human, Rat, Mouse, Goat, Sheep, PIG, FBS, Guinea Pig, Hamster, Monkey, and   Chicken serum IgG had less than 1% crossreactivity

Reactivity

Cow/Bovine